Re: [nmrpipe] inverse cosine tuned to 35 HNCACB
- You could do this with the NMRPipe adjustable sine-bell window "SP",
but NMRPipe has a related "window" function called JMOD, with parameters
specifically for coupling. So, multiplying by a 35 Hz cosine modulation
| nmrPipe -fn JMOD -j 35 -cos \
Add the "-inv" flag to divide by the coupling modulation instead.
This will only work if the coupling is small enough that its
modulation does not cross zero. Removing a coupling in this
way will also likely increase the noise level.
For general purpose application of j deconvolution, some have
have proposed using maximum entropy reconstruction. NMRPipe's
MEM can be used for this, but I've only done so with synthetic
JMOD: Options for J-Modulation Profile:
-off off [0.0] Modulation Start*PI. (Q1)
-j jHz [0.0] J-Modulation, Hz. (Q2)
-lb lbHz [0.0] Line Broadening, Hz. (Q3)
-sin Sine Modulation. (Q1 = 0.0)
-cos Cosine Modulation. (Q1 = 0.5)
-size aSize [APOD] Apodize Length.
-start aStart  Apodize Start.
-c fScale  Point 1 Scale.
-one Outside = 1.
-hdr Use Q Values from Header.
-inv Invert Window.
Quoting "Kelly, Mark" <Mark.Kelly@...>:
> Dear nmrPipe group,
> I'm trying process the CaCb dimension of an HNCACB and would like to
> multiplying the t1 time domain with an inverse cosine tuned to 35 Hz
> to remove the JCa-Cb coupling (ref below). Can you tell me what this
> would look like in a pipe script?
> Many thanks, Mark
> Wittekind, M. & Mueller, L. (1993). "HNCACB, a High-Sensitivy 3D NMR
> Experiment to Correlate Amide-Proton and Nitrogen Resonances with
> the Alpha- and Beta-Carbon Resonances in Proteins". J. Magn. Reson.,
> Series B. B101: 201-205.