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Projection reconstruction triple resonance experiments for Bruker spectrometers

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  • Winston Wen-Jin Wu
    Dear Bruker users, If you have TopSpin 1.3 or above, and you are interested in setting up triple resonance projection-reconstruction NMR on Bruker
    Message 1 of 6 , Oct 26, 2007
    • 0 Attachment

      Dear Bruker users,

       

      If you have TopSpin 1.3 or above, and you are interested in setting up triple resonance projection-reconstruction NMR on Bruker spectrometers. You can download our pulse sequences from the following URL.

      http://www.nmr.sinica.edu.tw/~winston/PR-PP/

      In this URL, you can also find an instruction file for the set up and processing of PR-NMR under Topspin. The setup and processing macros for PR-NMR can be obtained from Dr. Wolfgang Bermel of Bruker Inc. at wolfgang.bermel@...

                 I am not associated with Bruker Inc.. I just would like to share some PR-NMR pulse sequences that may be useful to the Bruker users.

                

      Best wishes,

       

      Winston

       

      PS, hi Frank, sorry, this has nothing to do with nmrpipe, unless someone decides to write processing macros for Bruker PR-NMR data, but the macros for them are already available for TopSpin. Unless someone would like to use different modes of reconstruction-routines and write the routines in his/her preferred computer language.

       

      ________________________________________________________

      Wen-Jin (Winston) Wu, PhD
      Assistant Research Specialist

      High-Field Nuclear Magnetic Resonance Center

      Institute of Biomedical Sciences,

      Academia Sinica, Taipei , Taiwan

      Tel: 886-2-2652-3549;            Email: winston@...

      http://www.nmr.sinica.edu.tw/~winston/
      ________________________________________________________


      From: nmrpipe@yahoogroups.com [mailto:nmrpipe@yahoogroups.com] On Behalf Of winston
      Sent: Saturday, October 13, 2007 3:21 AM
      To: nmrpipe@yahoogroups.com
      Subject: Re: [nmrpipe] Projection reconstruction

       

      Dear all,

       

      I thought the Bruker users might be interested in this.  If you are interested in acquiring triple resonance experiments using projection-reconstr uction NMR on Bruker spectrometers (and if you have Topspin 1.3 or above), please contact me at  winston@ibms. sinica.edu. tw. I will be happy to distribute the PR-pulse sequences that I have worked out: backbone and Cb triple resonance PR-NMR experiments with options for 15N-constant time evolution, semi-constant time evolutions, TROSY or 2H decoupling. The setup and processing macros can be obtained from Bruker. I will also put the pulse sequences onto our website in the near future.

       

      Best and cheerful regards,

       

      Winston

      ____________ _________ _________ _________ _________ ________

      Wen-Jin (Winston) Wu, PhD
      Assistant Research Specialist

      High-Field Nuclear Magnetic Resonance Center

      Institute of Biomedical Sciences,

      Academia Sinica, Taipei , Taiwan

      Tel: 886-2-2652-3549;            Email: winston@ibms. sinica.edu. tw

      ____________ _________ _________ _________ _________ ________

      On Fri, 12 Oct 2007 09:50:44 -0700, Shaheen Shojania wrote

      > Hi S
      lang=EN-US>astien,
      >
      > Most of the recent Biopack sequences are set up to do the PR method and in
      the sequence instructions there are tips to how to process using the varian software. As for processing the data with NMRPipe, you can process the individual planes but you cannot currently carry out the reconstruction. I think you also need a program to pre-process the fid's before running NMRPipe. To separate your planes and rconstruct the nD data set, you may want to check out Pei Zhou's (Duke university) website. His group has a program for doing the reconstruction using a number of different data formats (NMRPipe included). I found the program very useful.
      >
      > That said, I caution you on spending a lot of time on this approach unless
      your spectra is really well dispersed, has very good signal to noise, and you have a very good spectrometer. Otherwise, you may find that you need to collect the same number of tilted planes as you would conventional increments.
      >
      > Best of luck.
      >
      > Shaheen   
      >
      > On
      IsLunarDate="False" Day="7" Month="11" Year="2010" w:st="on">10/11/07 , Sebastien Morin <sebastien.morin. 1@ulaval. ca
      > wrote:


      >
      > Hi
      >
      > I would like to try projection reconstruction in Varian.
      >
      > E.Kupce and R.Freeman, J. Amer. Chem. Soc., vol. 125, pp.
      > 13958-13959 (2003).
      > E.Kupce and R.Freeman, J. Amer. Chem. Soc., vol. 126, pp.
      > 6429-6440 (2004).
      >
      > I wonder if my preferred processing program can deal with this kind of
      > data and how...
      >
      > Could anyone help me ?
      >
      > Thanks !
      >
      > S
      astien Morin
      >
      > --
      > ____________ _________ _________ ________
      > ____________ _________ _________ _________ ________
      > | |
      > || Sebastien Morin ||
      > ||| Etudiant au PhD en biochimie |||
      > |||| Laboratoire de resonance magnetique nucleaire ||||
      > ||||| Dr Stephane Gagne |||||
      > |||| CREFSIP (Universite Laval, Quebec, CANADA) ||||
      > ||| 1-418-656-2131 #4530 |||
      > || ||
      > |___________ _________ _________ _________ _________ |
      > ____________ _________ _________ ________
      >
      >


      >

      > --
      > Shaheen Shojania
      > c/o Lawrence McIntosh Lab
      > Department of Biochemistry and Molecular Biology
      > University of British Columbia
      > Room 4.555 Life Sciences Centre
      > 2350 Health Sciences Mall
      > Vancouver BC Canada    V6T 1Z3
      >
      > email: shojania@chem. ubc.ca or shojania@gmail. com
      > Tel: (604) 822-3029

    • Winston Wen-Jin Wu
      Dear Bruker users: A follow up to my previous email on the availability of Bruker projection reconstruction pulse sequences. I have just put up two more suits
      Message 2 of 6 , Nov 14, 2007
      • 0 Attachment

        Dear Bruker users:

         

        A follow up to my previous email on the availability of Bruker projection reconstruction pulse sequences. I have just put up two more suits of projection reconstruction (PR) pulse sequences: PR-TROSY-triple resonance and PR-TROSY-triple resonance with 2H decoupling. They were all tested, and ran fine.

        You can download our pulse sequences from the following URL.

        http://www.nmr.sinica.edu.tw/~winston/PR-PP/

         

        Cheerful regards,

         

        Winston

        ________________________________________________________

        Wen-Jin (Winston) Wu, PhD
        Assistant Research Specialist

        High-Field Nuclear Magnetic Resonance Center

        Institute of Biomedical Sciences,

        Academia Sinica, Taipei , Taiwan

        Tel: 886-2-2652-3549;            Email: winston@...

        http://www.nmr.sinica.edu.tw/~winston/
        ________________________________________________________

         


        From: Winston Wen-Jin Wu [mailto: winston@... .tw]
        Sent: Saturday, October 27, 2007 1:09 PM
        To: nmrpipe@yahoogroups.com
        Cc: 'Winston Wen-Jin Wu'
        Subject: Projection reconstruction triple resonance experiments for Bruker spectrometers

         

        Dear Bruker users,

         

        If you have TopSpin 1.3 or above, and you are interested in setting up triple resonance projection-reconstruction NMR on Bruker spectrometers. You can download our pulse sequences from the following URL.

        http://www.nmr.sinica.edu.tw/~winston/PR-PP/

        In this URL, you can also find an instruction file for the set up and processing of PR-NMR under Topspin. The setup and processing macros for PR-NMR can be obtained from Dr. Wolfgang Bermel of Bruker Inc. at wolfgang.bermel@...

                   I am not associated with Bruker Inc.. I just would like to share some PR-NMR pulse sequences that may be useful to the Bruker users.

                  

        Best wishes,

         

        Winston

         

        PS, hi Frank, sorry, this has nothing to do with nmrpipe, unless someone decides to write processing macros for Bruker PR-NMR data, but the macros for them are already available for TopSpin. Unless someone would like to use different modes of reconstruction-routines and write the routines in his/her preferred computer language.

         

        ________________________________________________________

        Wen-Jin (Winston) Wu, PhD
        Assistant Research Specialist

        High-Field Nuclear Magnetic Resonance Center

        Institute of Biomedical Sciences,

        Academia Sinica, Taipei , Taiwan

        Tel: 886-2-2652-3549;            Email: winston@...

        http://www.nmr.sinica.edu.tw/~winston/
        ________________________________________________________


        From: nmrpipe@yahoogroups.com [mailto: nmrpipe@yahoogroups.com ] On Behalf Of winston
        Sent: Saturday, October 13, 2007 3:21 AM
        To: nmrpipe@yahoogroups.com
        Subject: Re: [nmrpipe] Projection reconstruction

         

        Dear all,

         

        I thought the Bruker users might be interested in this.  If you are interested in acquiring triple resonance experiments using projection-reconstr uction NMR on Bruker spectrometers (and if you have Topspin 1.3 or above), please contact me at  winston@ibms. sinica.edu. tw. I will be happy to distribute the PR-pulse sequences that I have worked out: backbone and Cb triple resonance PR-NMR experiments with options for 15N-constant time evolution, semi-constant time evolutions, TROSY or 2H decoupling. The setup and processing macros can be obtained from Bruker. I will also put the pulse sequences onto our website in the near future.

         

        Best and cheerful regards,

         

        Winston

        ____________ _________ _________ _________ _________ ________

        Wen-Jin (Winston) Wu, PhD
        Assistant Research Specialist

        High-Field Nuclear Magnetic Resonance Center

        Institute of Biomedical Sciences,

        Academia Sinica, Taipei , Taiwan

        Tel: 886-2-2652-3549;            Email: winston@ibms. sinica.edu. tw

        ____________ _________ _________ _________ _________ ________

        On Fri, 12 Oct 2007 09:50:44 -0700, Shaheen Shojania wrote

        > Hi S
        lang=EN-US>astien,
        >
        > Most of the recent Biopack sequences are set up to do the PR method and in
        the sequence instructions there are tips to how to process using the varian software. As for processing the data with NMRPipe, you can process the individual planes but you cannot currently carry out the reconstruction. I think you also need a program to pre-process the fid's before running NMRPipe. To separate your planes and rconstruct the nD data set, you may want to check out Pei Zhou's (Duke university) website. His group has a program for doing the reconstruction using a number of different data formats (NMRPipe included). I found the program very useful.
        >
        > That said, I caution you on spending a lot of time on this approach unless
        your spectra is really well dispersed, has very good signal to noise, and you have a very good spectrometer. Otherwise, you may find that you need to collect the same number of tilted planes as you would conventional increments.
        >
        > Best of luck.
        >
        > Shaheen   
        >
        > On
        IsLunarDate="False" Day="7" Month="11" Year="2010" w:st="on">10/11/07 , Sebastien Morin <sebastien.morin. 1@ulaval. ca
        > wrote:


        >
        > Hi
        >
        > I would like to try projection reconstruction in Varian.
        >
        > E.Kupce and R.Freeman, J. Amer. Chem. Soc., vol. 125, pp.
        > 13958-13959 (2003).
        > E.Kupce and R.Freeman, J. Amer. Chem. Soc., vol. 126, pp.
        > 6429-6440 (2004).
        >
        > I wonder if my preferred processing program can deal with this kind of
        > data and how...
        >
        > Could anyone help me ?
        >
        > Thanks !
        >
        > S
        astien Morin
        >
        > --
        > ____________ _________ _________ ________
        > ____________ _________ _________ _________ ________
        > | |
        > || Sebastien Morin ||
        > ||| Etudiant au PhD en biochimie |||
        > |||| Laboratoire de resonance magnetique nucleaire ||||
        > ||||| Dr Stephane Gagne |||||
        > |||| CREFSIP (Universite Laval, Quebec, CANADA) ||||
        > ||| 1-418-656-2131 #4530 |||
        > || ||
        > |___________ _________ _________ _________ _________ |
        > ____________ _________ _________ ________
        >
        >


        >

        > --
        > Shaheen Shojania
        > c/o Lawrence McIntosh Lab
        > Department of Biochemistry and Molecular Biology
        > University of British Columbia
        > Room 4.555 Life Sciences Centre
        > 2350 Health Sciences Mall
        > Vancouver BC Canada    V6T 1Z3
        >
        > email: shojania@chem. ubc.ca or shojania@gmail. com
        > Tel: (604) 822-3029

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