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Lymphocyte gating of peripheral blood

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  • Janice and Ben
    Eur J Med Res 1996 Jan 19;1(4):215-222 Lymphocyte gating of peripheral blood in patients with leukemic low-grade non-Hodgkin s lymphoma by multiparametric flow
    Message 1 of 1 , Feb 8, 1998
      Eur J Med Res 1996 Jan 19;1(4):215-222

      Lymphocyte gating of peripheral blood in patients with
      leukemic low-grade non-Hodgkin's lymphoma by
      multiparametric flow cytometry.

      Hoffkes HG, Schmidtke G, Schmucker U, Brittinger G

      Division of Hematology, Department of Medicine, University of Essen, Germany.

      The standardized fluorescence intensity as expressed in molecules of
      equivalent soluble fluorescence (MESF) of lymphocytes from normal
      individuals and patients suffering from low-grade non-Hodgkin's-lymphomas
      was obtained comparing different staining patterns of CD45(FITC) and
      CD20(PerCP). After standardization of the flow cytometer using standardized
      fluorescent particles ('beads') significant differences could be obtained
      for hairy cell leukemia, chronic lymphocytic leukemia and immunocytomas in
      the peripheral blood. In contrast, centroblastic-centrocytic as well as
      centrocytic lymphomas showed no significant variations as compared to
      normal peripheral blood lymphocytes. According to these results, a new
      lymphocyte
      gating procedure was established by adding CD14(PE) and three-color
      measurement by CD45/CD14/CD20 staining of peripheral blood using
      erythrocyte lysis. The established gating procedure leads to a crucial
      discrimination and quantification of abnormal and normal lymphocytes per
      one measurement, whereas the 'leucogate' as defined by CD45/CD14 staining
      alone was insufficient for correct lymphocyte gate setting. In conclusion,
      the different staining of CD45 and CD20 in leukemic peripheral blood should
      be considered when fluorescence intensity or atypical peaks occurred in
      flow cytometric histograms suggesting for abnormal cell populations. In
      addition, it is possible to use this information to classify low-grade
      lymphomas.

      PMID: 9445762, UI: 98107486
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