interpretting TWINNED Rfree/R from CNS
- View SourceHi all,
Sorry for the non-CCP4 question.
I've refined my structure using detwinned data in CNS (version 1.1,
using the xxxxxx_xxxxxxx_twin.inp scripts under "Twinning" utilities,
after anneal_twin and bindividual_twin) TWINNED Rfree/R of 25/16
(sg=R3, twin frac =0.41, i posted questions about this earlier)
looking at the phi-psi plot, i have to do a little bit more model
my question is this twinned R/Rfree to be taked as final values ? or
should something else to be done after this ?
the ave. b-factor is 56. and many loop residues have b-factors in the
range 70-110 !!!!!!!! how to deal with this high b-factors ??
I'd appreciate any suggestions.
Graduate Research Assistant
Dept. of Biochemistry/Biophysics
Texas A&M University
College Station, TX 77843