Re: [beemonitoring] Re: collecting pollen from bees
Thanks to everyone for all the great information on pollen collection!
Diane L. Larson
USGS Northern Prairie Wildlife Research Center
1561 Lindig St.
St. Paul, MN 55108
“It’s a poor sort of memory that only works backwards,” the Queen remarked.
- Lewis Carroll, Through the Looking-Glass
From: Laura Burkle <laura.a.burkle.adv08@...> To: firstname.lastname@example.org Date: 11/12/2009 09:01 AM Subject: [beemonitoring] Re: collecting pollen from bees Sent by: email@example.com
Sam and all,
I don't have too much more to add that others haven't already
mentioned. But I will put a plug in for using individual vials to
collect bees from which you'd like to get pollen from later for network
analysis, etc. We use small 3.5 dram vials from Thornton Plastics or
standard 1.7mL microtubes, depending on the size of the bee. One can
carry a lot of these vials in the field, and you won't risk pollen
"contamination" between bees like you might in kill jars. We freeze the
bees in a cooler while we're in the field, and later process the dead
bees for pollen, rubbing the bee with a fuchsin-gelatin cube or washing
the bee in ethanol and staining the pollen with the fuchsin dye once the
ethanol has evaporated from the slide.
As for marking, we use different colors of Deco paint pens (extra
fine or fine tip) on the thorax. Paint pen dots seem to last at least
three weeks. We use a bee-squeezer (film canister or other vial with
the bottom cut out, mesh covering over the top, and a foam plunger to
push the bee against the mesh) to position the bee and mark through the
Postdoctoral Research Associate
Washington University in St. Louis