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Re: insect rehydration

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  • Márcia Maués
    Dear fellows, I have some orchid bees in my fridge (unfortunatelly forgoten...) that are extremely dry. We will need to remove the pollen grains attached to
    Message 1 of 1 , Sep 2, 2009
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      Dear fellows,

      I have some orchid bees in my fridge (unfortunatelly forgoten...) that
      are extremely dry. We will need to remove the pollen grains attached to
      their bodies, before pinning, using Acetic Acid, but we are afraid they
      will break into pieces... Is there any way to soften these bees before
      putting them into de Acetic Acid? Should I use a simple wet chamber?
      Best regards,

      Márcia

      --
      Márcia Motta Maués
      Embrapa Amazônia Oriental
      Laboratório de Entomologia
      Trav. Dr. Enéas Pinheiro s/n
      66.095-100 Belém, Pará, Brasil
      tel: + 55 (91) 3204-1033 / 1077
      fax: + 55 (91) 3276-9845


      K. W. Philip escreveu:
      > It is possible to relax insects in a few seconds with the
      > following simple procedure:
      >
      > 1) Apply a few drops of ethanol to the end of the abdomen.
      > Chitin is comparatively impervious to moisture--but the
      > alcohol allows the water to penetrate.
      >
      > 2) Immerse the end of the abdomen in water just below the
      > boiling point. In the case of butterflies, 10 to 20 seconds of
      > immersion will relax the genitalia. A couple of minutes
      > immersion will relax the entire body (even the antennae).
      >
      > I have not tried this with beetles, but it should work.
      > A short immersion to relax the abdomen should have very
      > little effect on the DNA in the legs. The alcohol speeds up
      > the absorption of water remarkably!
      >
      > Ken Philip
      >
      >

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