830Re: [beemonitoring] collecting pollen from bees
- Nov 10, 2009Hi Diane,
I have collected this kind of data for my dissertation and use methods put
together though talking with several other researchers who use similar
techniques. I bring about 10 collecting vials into the field and line
them with paper baggies (nothing fancy, I roll and fold up squares of
paper around the end of my finger). When they're filled, I close and
label the bags and re-line the tubes. You've still got to carry all those
baggies but it should be easier than a lot of vials. Or you could
transfer specimens to little pill boxes.
I use the fuchsin jelly method, the key is that you don't need a lot of
jelly! The hardest part is learning to or getting someone to id the
Feel free to email me off-list for more details,
> Hi all,--
> Sam Droege and I will be starting a pollination network study at Badlands
> National Park next summer. As part of this study, we want to collect
> pollen carried by individual bees as an indication of which flowers they
> have been visiting prior to collection (see Bosch et al. 2009 Ecol.
> Letters 12: 409-418). We have two questions. First, is there an
> alternative to individual kill vials, given that we want insect-specific
> pollen information? Working in the backcountry, it will be difficult to
> handle the number of vials we'll likely need. Second, in removing pollen
> from the insects' bodies, is the method described in Kearns and Inouye
> 1993, i.e., rubbing a small cube of fuchsine stained gelatin over the
> insect's body, still the best method? If so, how soon must this be done
> after capture?
> If anyone has experience with mutualist networks in general, I'd love to
> talk with you.
> Thanks for any advice!
> Diane L. Larson
> Research Biologist
> USGS Northern Prairie Wildlife Research Center
> 1561 Lindig St.
> St. Paul, MN 55108
> Voice 651-649-5041
> FAX 651-649-5040
> Email: dlarson@...
> ?It?s a poor sort of memory that only works backwards,? the Queen
> - Lewis Carroll, Through the Looking-Glass
Graduate Program in Ecology and Evolution
Dept. of Ecology, Evolution and Natural Resources
Environmental & Natural Resources Building
14 College Farm Road
New Brunswick, New Jersey 08901
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