2880Re: [beemonitoring] Identifying pollen collected from bees
- Aug 19, 2013The basic method is as Barbara indicates, you make a reference collection of flowering plants in your area which you use to compare with pollen you find on bees. Unfortunately, species, generic and sometimes even family level ids of pollen are often quite diffcult unless you acetolylze your pollen, a rather nasty process which cleans up the grains the pollenkit so you can see the fine details of the exine. SEM is now a very common way to go but again acetolysis is usually used to prepare the pollen. Details and references are in "Practical Pollination Biology" by Dafni et al, 2005, Enviroquest Ltd and the older "Techniques for Pollination Biologists" by Kearns and Inouye, 1993 University of Colorado Press.bestJackJohn L. Neff
Central Texas Melittological Institute
7307 Running Rope
Austin,TX 78731 USA
From: "barbara.abraham@..." <barbara.abraham@...>
To: lar322@...; firstname.lastname@example.org
Sent: Monday, August 19, 2013 8:48 AM
Subject: RE: [beemonitoring] Identifying pollen collected from bees
Laura,We did it by collecting the pollen from blooming flowers and comparing it with the pollen on bees we collected on the flowers. The recipe for the gel with stain is in Inouye's pollen methods book.BarbBarbara J Abraham, PhDAssociate ProfessorSEEDS Chapter AdvisorDepartment of Biological SciencesHampton UniversityHampton, Va 23668757-727-5283From: email@example.com [firstname.lastname@example.org] on behalf of Laura Russo [lar322@...]
Sent: Sunday, August 18, 2013 11:05 AM
To: Bee United
Subject: [beemonitoring] Identifying pollen collected from bees
Dear all,Laura Russo
I am interested in identifying pollen samples collected from bees. I have looked at some resources on the internet, but I thought I would probe this listserve to see if anyone has had experience with that process, and therefore recommendations for resources (i.e. books, websites, people, etc.).
Any advice on the collection methodology would also be gratefully received.
Thank you very much for your time,
Intercollege Graduate Degree Program in Ecology
Pennsylvania State University
University Park, PA 16802
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