Stitching a 32646 x 27955 image
- In an experimental work I've made a very large panorama composed from
230 photos. The PTGui software was very good in handling all the images
in the stitching process. Details regarding the workflow can be found
in the webpage:
Comments and questions are welcome.
- On Jul 31, 2007, at 1:28 PM, rox_ana_mustata wrote:
> In an experimental work I've made a very large panorama composed fromThat is a fascinating experiment you have done. I assume that you
> 230 photos. The PTGui software was very good in handling all the
> in the stitching process. Details regarding the workflow can be found
> in the webpage:
> Comments and questions are welcome.
were moving the sample about, making rows and columns of photos.
This seems similar to the type of work we have seen where someone
photographs a street or a mural to create a "linear panorama", except
you have the added complexity of multi-row photography! Folks who
make linear panoramas, if I recall correctly, have to do a lot of
work because of the shifting of the camera and the resulting parallax
problems. At least here you don't have the degree of depth issues
(like side streets) that the usual linear pano must deal with.
If I am understanding properly what you did, it is remarkable that
PTGui handled it so well on "automatic"! You also seem to have very
good registration between the different color mosaics. That is
surprising, since you shot each color separately it seems. How did
you combine the colors to make the composite image? (Also, what kind
of tissue was it?)
Excellent, interesting work,
John C. Riley, Ph.D.
Assoc. Prof. of Physics
Univ. of SC Upstate
and 4Pi-VR media solutions
[Non-text portions of this message have been removed]
- The sample was moved under the microscope objective. For each move,
three photos were acquired (blue, red, and green color, corresponding
to three fluorescence filter sets). The image acquisition was a pain in
the ass, taking 3 days (with an average of ~5 hours daily). As you can
see, should be 231 images for each color (one image is missing, look in
the middle left part, of the section).
First I've tried with PTGui 6 and a lot of pictures were not correctly
positioned. PTGui 7 is better regarding the control points system.
The combined image was obtained by layering the images in PS. The upper
layers were set to difference, resulting in combination of colors (for
example, green + red makes yellow).
The sample was a section from a human mole. The medical diagnostic is
not relevant here. The coloring procedures done here are only
- rox_ana_mustata wrote:
>I guess the only realistic option is 10 rows of 23 images each.
> In an experimental work I've made a very large panorama composed from
> 230 photos.
The aspect-ratio of the final image implies a lot of horizontal
overlap and hardly any vertical overlap.
Is that correct ?
What is the NA of the x20 objective, the relay lens (or
eyepiece/camera lens combination) magnification and pixel-size of the
camera sensor ?
> Comments and questions are welcome.There is a low-resolution web image but no link for the cropped
- Is not such simple There were 22 rows, having from 1 image (row 1,
the top) to 13 images.
As far as I remember the objective NA is 0.75. The microscope is a
Nikon E600 with a DS-U1 digital camera. Take a look at
for technical specification.
I updated the webpage with a 100% crop image from the composite .jpg
file (21408 x 25000 pixels).
--- In PanoToolsNG@yahoogroups.com, "David Sykes" <killspammers2@...>
> I guess the only realistic option is 10 rows of 23 images each.the
> The aspect-ratio of the final image implies a lot of horizontal
> overlap and hardly any vertical overlap.
> Is that correct ?
> What is the NA of the x20 objective, the relay lens (or
> eyepiece/camera lens combination) magnification and pixel-size of
> camera sensor ?
> There is a low-resolution web image but no link for the cropped
> high-resolution image.
> David Sykes
- Roxana wrote:
> There were 22 rows, having from 1 image ... to 13 images.I make that 286 images, not 230 :-)
> The microscope is a Nikon E600 with a DS-U1 digital camera.Thanks, but that is the camera control unit.
Which camera head is it ?