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Hepatitis C Virus RNA Assays: A Comparison of SuperQuant and Monitor

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  • claudine intexas
    Hepatitis C Virus RNA Assays A Comparison of SuperQuant and Monitor Emilia Hadziyannis, M.D.; Alexandros Hadziyannis, M.D.; Belinda Yen-Lieberman, Ph.D.;
    Message 1 of 1 , Jul 30, 2001
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      Hepatitis C Virus RNA Assays
      A Comparison of SuperQuant and Monitor




      Emilia Hadziyannis, M.D.; Alexandros Hadziyannis,
      M.D.; Belinda Yen-Lieberman, Ph.D.; Michelle L. Kiwi,
      M.P.H.; Sandra Hodnick, R.N.; Fotini Spanou, M.T.;
      Colleen Starkey, M.T.; Zobair M. Younossi, M.D.,
      M.P.H.

      From the Academic Department of Medicine and National
      Center for Communicable Diseases of the Liver (E.H.,
      A.H., F.S.), Hippokration General Hospital, Athens,
      Greece; the Departments of Gastroenterology (M.L.K.,
      S.H., Z.M.Y.) and Clinical Pathology (E.H., B.Y-L.,
      C.S.), The Cleveland Clinic Foundation, Cleveland,
      Ohio, U.S.A.; and the Center for Liver Diseases
      (Z.M.Y.), Inova Fairfax Hospital, Falls Church,
      Virginia, U.S.A.

      JOURNAL OF CLINICAL GASTROENTEROLOGY 2001;33:45-48


      --------------------------------------------------------------------------------

      Abstract

      Hepatitis C RNA testing has been used extensively to
      assess the efficacy of antiviral therapy and has
      increasingly become an integral part of clinical
      management of patients with chronic hepatitis C. A
      variety of commercially available hepatitis C virus
      (HCV) RNA tests are used to detect HCV RNA
      qualitatively or quantitatively. These commercial
      tests have fundamental differences that are reflected
      on the values they generate. We compared two widely
      used assays, HCV SuperQuant (SQ) and Amplicor HCV
      Monitor (M1 and M2), in sera of patients with chronic
      hepatitis C. A total of 506 sera from 79 patients were
      tested with both assays. The data were logarithmically
      transformed and analyzed by linear regression and
      measurement of agreement. Two hundred thirty-eight
      sera had HCV RNA values within the dynamic range of
      both assays. The correlation between the assays was
      fair, with a correlation coefficient (r) of 0.699.
      Overall, SQ generated higher values than M1 with a
      mean difference of 0.558 log (SD = 0.624). One hundred
      ninety-four (38%) and 121 (24%) of the sera were below
      the dynamic range of M1 and SQ, respectively.
      Seventy-three sera, undetectable by M1, were positive
      by SQ. The Amplicor HCV Monitor 2.0 (M2) was performed
      in 66 sera. All were positive by SQ and M2, but only
      38 were within the dynamic range of M1. The
      correlations between these tests were good (r =
      0.68�0.78), but the agreement was rather poor. In
      conclusion, this study confirms that both SQ and M2
      are more sensitive than M1. Additionally, our results
      show rather poor agreements between these assays. The
      recent attempts in standardizing the reporting of
      these assays should make their results more easily
      interchangeable.

      Key Words: HCV RNA; Viral load; Polymerase chain
      reaction quantification


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