Re: [GIWorld-Hepatitis] How Long Does HCV Live Outside of the Body?
- Found this in HepC File......thought I'd pass it on. Hope this answers your
Summarized from a report by Kris Krawczynski, Miriam J. Alter, Betty H.
Robertson, Ling Lu, Jon E. Spelbring, Karen A. McCaustland, Centers for Disease
Control and PRevention, Atlanta, GA--Background epidemiologic studies of HCV have
indicated that transmission among patients in health care settings is
associated with contaminated vehicles such as multi-dose medication vials and re-used
needles and syrings among injecting drug users is associated with
contaminated drug paraphernalia such as cookers and cotton. Determining the viability of
HCV, and extent to which the infectios virus can sruvive on environmental
surfaces, is critical for developing effective recommendations for prevention and
Three samples of chimpanzee blood containing HCV genotype 1a were dried for
16 hours. One vial was rehydrated and the other two were stored in a
controlled environment, one for 4 days the other for 7 days, and then rehydrated. All
were frozen for use later. A test chmpanzee was given equal sized infectious
doses of each sample throughout the study. The chimp's blood was tested for
HCV RNA, HCV antibodies, and ALT twice weekly. Liver biopsies were obtained
weekly or biweekly and tested for virus antigen and histopathology.
The animal was first inoculated with the sample that was dried/stored for
7days and followed during 129 days. Subsequently, the animal was inoculated with
the sample that was dried/stored for 4 days and followed for 134 days, and
finally inoculated with the sample dried overnight and followed for 201 days.
Virologic, serologic, and clinical data from three control chimpanzees
inoculated with a sample of untreated HCV-positive blood were included in the study.
HCV RNA was detectable in the dried blood, but a ten-fold decrease of
detectable HCV RNA was found in the dried samples compared with the HCV RNA titer of
the original, untreated HCV positive blood. No evidence of HCV infections was
detected in the test of chimpanzee after inoculation with either the 7-day or
4-day dried/stored samples. All serum samples tested were negative for HCV
RNA and anti-HCV; ALT activity level remained in the normal range. However,
after inoculation with the overnight dried sample. HCV RNA was detected in serum
of the chimp from day 7 post-inoculation, and the viral load reeached 6.0to
7.3 logs IU/mL. HCVAg positive hepatocytes were observed from day 11 post
inoculation, seroconversion to anti-HCV was observed on day 127, and the animal
was still positive for HCVRNA (4.8 logs IU/mL) at day 201 post-infection. ALT
activity level was elevated over the normal range from day 11 post-inoculation
and remained elevated until the end of the observation. Virologic, serologic,
and clinical evidence of HCV infection and acute hepatitis was found in all
three control animals.
Studies in a chimpanzee suggest that HCV may survive on environmental
surfaces at room temperature at least 16 hours but not longer than 4 days. The
potential for HCV to survive in the environment re-emphasizes the importance fo
cleaning and disinfecting.
[Non-text portions of this message have been removed]