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Re: [GIWorld-Hepatitis] How Long Does HCV Live Outside of the Body?

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  • AVansi7465@aol.com
    Found this in HepC File......thought I d pass it on. Hope this answers your question. Anne Summarized from a report by Kris Krawczynski, Miriam J. Alter,
    Message 1 of 1 , Apr 1 3:56 PM
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      Found this in HepC File......thought I'd pass it on. Hope this answers your
      question. Anne

      Summarized from a report by Kris Krawczynski, Miriam J. Alter, Betty H.
      Robertson, Ling Lu, Jon E. Spelbring, Karen A. McCaustland, Centers for Disease
      Control and PRevention, Atlanta, GA--Background epidemiologic studies of HCV have
      indicated that transmission among patients in health care settings is
      associated with contaminated vehicles such as multi-dose medication vials and re-used
      needles and syrings among injecting drug users is associated with
      contaminated drug paraphernalia such as cookers and cotton. Determining the viability of
      HCV, and extent to which the infectios virus can sruvive on environmental
      surfaces, is critical for developing effective recommendations for prevention and
      control.

      Three samples of chimpanzee blood containing HCV genotype 1a were dried for
      16 hours. One vial was rehydrated and the other two were stored in a
      controlled environment, one for 4 days the other for 7 days, and then rehydrated. All
      were frozen for use later. A test chmpanzee was given equal sized infectious
      doses of each sample throughout the study. The chimp's blood was tested for
      HCV RNA, HCV antibodies, and ALT twice weekly. Liver biopsies were obtained
      weekly or biweekly and tested for virus antigen and histopathology.

      The animal was first inoculated with the sample that was dried/stored for
      7days and followed during 129 days. Subsequently, the animal was inoculated with
      the sample that was dried/stored for 4 days and followed for 134 days, and
      finally inoculated with the sample dried overnight and followed for 201 days.
      Virologic, serologic, and clinical data from three control chimpanzees
      inoculated with a sample of untreated HCV-positive blood were included in the study.

      Results:

      HCV RNA was detectable in the dried blood, but a ten-fold decrease of
      detectable HCV RNA was found in the dried samples compared with the HCV RNA titer of
      the original, untreated HCV positive blood. No evidence of HCV infections was
      detected in the test of chimpanzee after inoculation with either the 7-day or
      4-day dried/stored samples. All serum samples tested were negative for HCV
      RNA and anti-HCV; ALT activity level remained in the normal range. However,
      after inoculation with the overnight dried sample. HCV RNA was detected in serum
      of the chimp from day 7 post-inoculation, and the viral load reeached 6.0to
      7.3 logs IU/mL. HCVAg positive hepatocytes were observed from day 11 post
      inoculation, seroconversion to anti-HCV was observed on day 127, and the animal
      was still positive for HCVRNA (4.8 logs IU/mL) at day 201 post-infection. ALT
      activity level was elevated over the normal range from day 11 post-inoculation
      and remained elevated until the end of the observation. Virologic, serologic,
      and clinical evidence of HCV infection and acute hepatitis was found in all
      three control animals.

      Conclusions:

      Studies in a chimpanzee suggest that HCV may survive on environmental
      surfaces at room temperature at least 16 hours but not longer than 4 days. The
      potential for HCV to survive in the environment re-emphasizes the importance fo
      cleaning and disinfecting.


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