...For those interested...
We have discovered several things since I sent this note:
* The Beckman AP96 20ul nonsterile tips only survive about 20-30 washes before performance degrades significantly (i.e. from 5% to 25% CV). We still use these, but only for limited wash applications
* We have reloaded the Beckman tips 20x with no real issues; Beckman says 15 loads max. This isn't much of an problem for us, since we only load 2-5x.
* We have tested 2 types of tips that Marsh supplies (#918-261 from MBP) and another type Marsh sells (#TN20R-AFX). The MBP tips work very well (5-10% CV's with our plasmid DNA's after washing) and can be washed up to 200x with little degradation in performance. The same is true for the AFX tips, but we have noticed quality issues with them (damaged/bent tips mostly)
* We also tested tips from USA scientific (#1061-2400). These work well also and can be washed up to 200x, but we have seen 1-2 tips in several racks that had mold problems (air bubbles) that caused pipetting failure.
The bottom line is that we are using the Beckman tips for miscellaneous uses that don't require many washes and the MBP tips for long methods with heavy wash requirements.
DuPont Crop Genetics
Trait & Technology Development
P.O. Box 6104
Newark DE 19711-6104
From: Jung, Mark T.
Sent: Wednesday, August 27, 2003 1:01 PM
Subject: [lrig-discussion] Biomek FX disposable tip wash
We have been doing some pipetting accuracy testing of disposable tips on our FX 96 channel pod (the AP96 P20 nonsterile tips -P/N BK717254). Does anyone out there have experience with this? I am interested in how repeated washes (using 40ul water in the Beckman 96 wash station) and tip loadings affects CV's. We would like to reuse tips which means several (10-20) loadings and many (100-500) washes. We have noticed decreased accuracy and precision after 100 washes or so. CV's using our normal techniques are 10-15% pipetting 2ul of plasmid DNA solution into a PCR plate with 5ul in it. This degrades to 30-50% or worse after ~200 washes and 10 loadings with some tips not transferring anything. We are working on identifying if loading or washing is more responsible for the degradation in performance. Any thoughts?
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