Hello, Our lab uses both as a matter of personal preference. I like H/E, though visually the X-mas Tree is at least equal, especially under normal light whereMessage 1 of 4 , Mar 1, 2006View SourceHello,
Our lab uses both as a matter of personal preference. I like H/E,
though visually the X-mas Tree is at least equal, especially under
normal light where the sperm are pretty easy to see. The reason I
prefer H/E is the staining time is much shorter. Sitting around 15
minutes plus waiting for X-mas Tree slides to stain is too long.
Though our staining time with X-mas Tree may be because of the
dilute stain our protocol calls for. Others with experience outside
our lab have said they used a more concentrated stain with a shorter
staining time. As for the end result of what you see... I don't
personally believe there is much of a difference. I think it is a
preference of the eye. One other issue I can think of is that some
people don't seem to see sperm stained with H/E under phase the
same. I see nice little green, almost glowing orbs. Some say they
only see a subdued yellowish color. This could be harder to spot in
a crowded field of view. Maybe then X-mas Tree would be better.
Hope that helps,
Los Angeles County Sheriff Crime Lab
--- In email@example.com, "dajw101" <dajw101@...>
> Dear all,
> I work in a Forensic Science Laboratory in the UK. We currently
> the Haemotoxylin/Eosin staining for use with the indentificationof
> spermatozoa in sexual assault cases etc.
> I am interested in comparing the method that we use with the
> Christmas Tree staining technique.
> What are peoples experiences of the Christmas Tree staining
> What staining protocols do you use? and how red do the spermatozoa
> stain up? - the ones I have seen recently I would describe as pale
> red if not nearly pink.
> Have any individuals/labs out there switched from using
> Haemotoxylin/Eosin to Christmas tree or vice versa and if so why?
> Many thanks,
Dear David, I have used both Haematoxylin/Eosin and Christmas Tree staining for the identification of sperm. I believe the Christmas Tree is a little betterMessage 1 of 4 , Mar 1, 2006View SourceDear David,
I have used both Haematoxylin/Eosin and Christmas Tree staining for the
identification of sperm. I believe the Christmas Tree is a little
better than H&E. The following reference also believes the Christmas
Tree to be better than the H&E: J. P. Allery, N. Telmon, and R.
Mieusset, "Cytological Detection of Spermatozoa: Comparison of Three
Staining Methods," J. Forensic Science, Vol 46, 2001, pages 349-351.
The 2005 2nd edition of Volume II of Saferstein's Forensic Science
Handbook has a chapter titled: "The Identification of Semen and Other
Body Fluids" that addresses the history, chemistry (staining times) and
popularity of the Christmas Tree stain.
Our laboratory switched from H&E to Christmas Tree in the middle to
Ventura Sheriff's Crime Lab
>>> dajw101@... 2/28/2006 4:48:54 PM >>>Dear all,
I work in a Forensic Science Laboratory in the UK. We currently use
the Haemotoxylin/Eosin staining for use with the indentification of
spermatozoa in sexual assault cases etc.
I am interested in comparing the method that we use with the
Christmas Tree staining technique.
What are peoples experiences of the Christmas Tree staining
What staining protocols do you use? and how red do the spermatozoa
stain up? - the ones I have seen recently I would describe as pale
red if not nearly pink.
Have any individuals/labs out there switched from using
Haemotoxylin/Eosin to Christmas tree or vice versa and if so why?
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David, Our lab used to use the Christmas tree stain, but we switched to the Haemotoxylin/eosin stain for various reasons. I ve never personally used theMessage 1 of 4 , Mar 2, 2006View SourceDavid,
Our lab used to use the Christmas tree stain, but we switched to the Haemotoxylin/eosin stain for various reasons. I've never personally used the christmas tree stain as our protocol changed before I arrived, but I've spoken with some of the other analysts and they say that the haemotoxylin stain is easier to read and perform. We actually just had the opportunity/necessity to change from our standard haemotoxylin to a modified version or a new stain all together because the mercury in the preservative was being flagged by the EPA or some other environmental agency. Instead of reverting to one of the stains from the past, we searched to find a modified version - I ordered my premixed from Fisher. I don't know if that's any useful information for you or not, but that's what's happened here in our lab system.
Erika M Jones
Forensic Biologist II -- Western Regional Laboratory
"You are 87% water; the other 13% keeps you from drowning." - P. E. Morris.
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