Hi Ron,

You did not miss anything and have very good questions.? I asked the same
questions!? Initially I wanted to set up a genetic test to determine the level
of homogenization.? A simple test using a visible genetic marker or a more
involved molecular analysis.? Then, my wife, being the smarter one...suggested
using a simple dye.? At first, I was skeptical there was?significant mixing
taking place in the chamber, but two things changed that view.? One, there is
mechanical mixing that occurs during the semen collection process.? The plunger
is designed so that rotation provides a mechanical mixing inside the chamber.?
The pictures shown do not account for the mechanical mixing as?the pictures
indicate mixing after the semen collection was completed.? To determine if the
movement of the dye was simply due to the penetrating property of the dye, I
replicated the procedure using different diameters of tubing and found the
movement of the dye was not proportional to the diameter of the tubing/chamber
used.? Using standard 100ul semen storage tubes filled with semen the dye does
not penetrate more than 1-2mm, even after several days.?

I hope this answers your questions.

Best regards,
Joe


Joseph S. Latshaw, Ph.D.
Latshaw Apiaries
6050 Harlem Road
New Albany, Ohio 43054
USA
Ph: 614.855.9065
E-mail: ohioqb@...
Web: latshawapiaries.com


-----Original Message-----
From: Ron & Eefje <ron-eefje@...>
To: II-List@yahoogroups.com
Sent: Thu, 1 Jan 2009 10:44 am
Subject: Re: [II-List] New Instrumental Insemination Apparatus






Hi Joe,

Thanks for the post and a Happy New year back!

What I did not understand exactly was how you arrived at the conclusion
that there was in fact a homogenized mixture of semen 9,5 hrs after
having added some dye. Wasn't the penetrating property of the added dye
the reason for the spreading of the dye through the drawn up semen? And
if that is so then a homogenizing process of semen may not have taken
place or at least not exactly in line with the shown spreading of the
dye through the semen.
What mechanical part of the syringe is according to you promoting the
homogenizing process?
Did I miss something?

Ron van Mierlo
Sweden

Joseph S. Latshaw skrev:
> Hi Everyone,
>
> A little while back I mentioned that I have been working on a new instrument.?
With a little extra time over the holidays, I a nearly finished with the
assembly.? The new Latshaw Micro Instrument is a highly simplified and
economical design that features a large capacity semen homogenizing syringe.? I
am very pleased with this new instrument and syringe and just wanted to share
the news with everyone.? I have some pictures up on my website with a better
description for those?who are interested.
>
> Thanks and Happy New Year!
> Joe
>
>
> Joseph S. Latshaw, Ph.D.
> Latshaw Apiaries
> 6050 Harlem Road
> New Albany, Ohio 43054
> USA
> Ph: 614.855.9065
> E-mail: ohioqb@...
> Web: latshawapiaries.com
>
>
> [Non-text portions of this message have been removed]
>
>
> ------------------------------------
>
> Yahoo! Groups Links
>
>
>
>
>





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